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. Author manuscript; available in PMC: 2019 Mar 15.
Published in final edited form as: Bioorg Med Chem. 2017 Sep 6;26(6):1212–1219. doi: 10.1016/j.bmc.2017.09.002

Figure 4.

Figure 4

Design and diversity characterization of the library. The genetically-encoded library based on the RTD-1 scaffold was produced at DNA level by synthesizing a degenerate synthetic oligonucleotide template encoding the precursor RTD-1 polypeptide and using a NNK (where N = A, C, G or T and K = G or T) codon scheme for the randomized positions located in residues 6, 8, 9, 10, 11 and 13. The library was characterized by sequencing 60 randomly picked clones and using the WebLOGO algorithm.38 The picture shows the WebLOGO picture of the θ-defensin peptide segment where the mutations were introduced. The Cys residues were conserved to allow the corresponding peptides adopting a native θ-defensin fold.