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| Primary Antibody/Lectin (manufacture, catalog#, dilution used) |
Secondary Antibody (manufacture, catalog#; dilution used) |
|
| Pericyte Marker |
|
| Goat anti-mouse aminopeptidase N/ANPEP (CD13; R&D systems, AF2335; 1:100) |
Alexa fluor 488- or 568-conjugated donkey anti-goat (Invitrogen, A-11055 or A-11057; 1:500) |
|
| Goat anti-human platelet-derived growth factor receptor beta (PDGFRβ; R&D Systems, AF385; 1:100) |
Alexa fluor 568-conjugated donkey anti-goat (Invitrogen, A-11057; 1:500) |
|
| Fibrinogen/Fibrin |
|
| Rabbit anti-human fibrinogen (Dako, A0080; 1:500) |
Alexa fluor 568-conjugated donkey anti-rabbit (Invitrogen, A-10042; 1:500) |
|
| Myelin |
|
| Goat anti-human myelin basic protein (MBP; Santa Cruz, sc-13914-R; 1:500) |
Alexa fluor 488- or 568-conjugated donkey anti-goat (Invitrogen, A-11055 or A-11057; 1:500) |
|
| Axons |
|
| Mouse anti-mouse axonal SMI-312 neurofilament marker (SMI-312; BioLegend, SMI312; 1:500) |
Alexa fluor 568-conjugated donkey anti-mouse (Invitrogen, A-10037; 1:500) |
|
| Oligodendrocytes |
|
| Rabbit anti-mouse Olig2 (Millipore, AB9610; 1:200) |
Alexa fluor 488- or 568-conjugated donkey anti-rabbit (Invitrogen, A-21206 or A-10042; 1:500) |
|
| Monoclonal mouse anti-Olig2 (ThermoFisher, MA5-15810; 1:200) |
Alexa fluor 488- or 568-conjugated donkey anti-mouse (Invitrogen, A-21202 or A-10037; 1:500) |
|
| Mature Oligodendrocytes |
|
|
| Mouse anti-cyclic nucleotide phosphodiesterase (CNPase; Abcam, ab6319; 1:500) |
Alexa fluor 647-conjugated donkey anti-mouse (Invitrogen, A-31571; 1:500) |
|
| Oligodendrocyte Progenitor Cells |
|
|
| Rabbit anti-platelet-derived growth factor receptor alpha (PDGFRα; Cell Signaling, #3174; 1:200) |
Alexa fluor 568-conjugated donkey anti-rabbit (Invitrogen, A-10042; 1:500) |
|
| Neurons |
|
| Rabbit anti-mouse NeuN (Millipore, ABN78; 1:500) |
Alexa fluor 568- or 647-conjugated donkey anti-rabbit (Invitrogen, A-10042 or A-31573; 1:500) |
|
| Vasculature |
|
| Dylight 488-conjugated L. esculentum lectin (Vector Labs, DL-1174; 1:200) |
N/A |
|
| Microglia |
|
| Rabbit anti-mouse ionized calcium binding adaptor molecule 1 (Iba-1; Wako, 019-19741; 1:1,000) |
Alexa fluor 488-conjugated donkey anti-rabbit (Invitrogen, A-21206; 1:500) |
|
|
| Astrocytes |
|
| Rabbit anti-Glial Fibrillary Acidic Protein (GFAP; Dako, z0334; 1:500) |
Alexa fluor 647-conjugated donkey anti-rabbit (Invitrogen, A-31573; 1:500) |
|
| Flow Cytometry & In Vitro Mature Oligodendrocytes |
|
| Rabbit anti-proteolipid protein (PLP; Abcam, ab105784; 1:2,000) |
Alexa fluor 488-conjugated donkey anti-rabbit (Invitrogen, A-21206; 1:200) |
|
| Mouse anti-myelin basic protein (SMI-99; BioLegend; 1:500) |
Alexa fluor 647-conjugated donkey anti-mouse (Invitrogen, A-21202 or A-31571; 1:200) |
|