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. 2018 Mar 2;9:415. doi: 10.3389/fimmu.2018.00415

Figure 5.

Figure 5

CCR6 C-terminal Ser/Thr clusters affect CCL20-induced actin polymerization. (A) Jurkat cells stably expressing wild-type CCR6 (WT-CCR6) or various CCR6 mutants were stimulated with 100 ng/ml CCL20 for the indicated times, fixed by 4% formaldehyde, stained with phalloidin conjugated with Alexa 488, and subjected to flow cytometry analysis. (B) Jurkat cells stably expressing WT-CCR6 or various CCR6 mutants were seeded on 10 µg/ml fibronectin-coated coverslips and stimulated with 100 ng/ml CCL20 for 10 min, fixed by 4% formaldehyde, and stained with Alexa 488-conjugated phalloidin. Images were obtained on an LSM700 confocal microscope. Scale bar = 10 µm. (C) Percentage of polarized cells per field was calculated (n = 6–24). ***p < 0.001 compared with WT-CCR6.