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. 2018 Mar 2;9:65. doi: 10.3389/fgene.2018.00065

FIGURE 1.

FIGURE 1

Cc2d1b KO mice are viable and fertile and present normal anatomical development of the brain. (A) Gene trap containing engrailed two splice acceptor (En2SA) sequence followed by a β-Galactosidase cassette (βgal) and a neomycin resistance cassette (neo) is flanked by flippase recognition target (FRT) sites between exons 2 and 3. LoxP sites for Cre recombinase targeting flank exon 3. (B) 1bKO mice are born in predicted Mendelian ratios (number of pups indicated above in parentheses; data from 5 litters) and (C) are indistinguishable from WT and 1bHET mice. (D) Immunoblot analysis of CC2D1A and CC2D1B expression in WT and 1bKO mice. Normal levels of CC2D1A and a complete absence of CC2D1B are shown in the 1bKO mice. (E) The size and organization of the adult 1bKO brain is indistinguishable from WT brain stained with hematoxylin and eosin. Scale bar: 1 mm.