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. 2018 Mar 2;9:65. doi: 10.3389/fgene.2018.00065

FIGURE 2.

FIGURE 2

Cc2d1a/Cc2d1b double LOF is embryonic lethal, while double heterozygotes are viable. (A) Genotypes at postnatal day 0 (P0) of 124 pups resulting from 22 double heterozygous (1a/1b-dHET) crosses (number of pups for each genotype indicated above in parentheses). The 1a/1b double knockout (A–/– B–/–) pups were never found at P0. (B) Representative images of normal embryonic day 11.5 (E11.5) embryo with a single intact Cc2d1 allele (Left) and a double KO embryo (Right; arrow indicates empty yolk sac). Scale bars: 1 mm. (C) The size and organization of the adult 1a/1b-dHET brain is indistinguishable from wild-type mice stained with hematoxylin and eosin. Scale bar: 1 mm. (D) Immunoblot analysis of CC2D1A and CC2D1B expression in wild-type and 1a/1b-dHET mice. A half dose of each CC2D1 protein was found. Results expressed as mean ± SEM. ∗p < 0.05 (two tailed t-test).