Figure 7.

In vitro reconstitution of Cdv protein. (A) Cryo-EM microscopy of CdvA from S. acidocaldarius that polymerize on the other surface of a liposome made from tetraether polar lipid fraction (E). Scale bar - 50 nm. (B) 3D reconstruction of the CdvA filaments from (A). (C) Negatively stained EM of M. sedula CdvA double-helical polymers reconstituted in vitro that contained DNA. (D,E) Zooms of the emphasized areas indicated in panel (C). scale bars 20 nm. (F) Negatively stained EM image of CdvBΔC polymers (Residues 1–167 -ΔMIM domain) from M. sedula. (A,B) are reproduced from Dobro et al. (2013) with permission. (C–F) Are reproduced from Moriscot et al. (2011) with permission.