Table 1.

Selected D. discoideum mutants implicated in L. pneumophila interactions.

Host cell factor	Manipulation	Effects on infection:		References
		Uptake	Growth
ATG 9	Knockout	Down	Up	Tung et al., 2010
ATG 16	Knockout	Down	n.t.	Xiong et al., 2015
ATG 9/ATG 16	Double knockout	Down	n.t.	Xiong et al., 2015
Coronin	Knockout	Down	Up	Solomon et al., 2000; Fajardo et al., 2004
		n.t.	Up
Coronin 7	Knockout	Up	Normal	Shina et al., 2010
	Overexpression	Down	Normal
Coronin/coronin 7	Double knockout	Down	Up	Shina et al., 2011
Dd5P4 (OCRL)	Knockout	Down	Up	Weber et al., 2009a
PLC	Inhibitors	Down	Normal	Peracino et al., 2010
PI3K 1/2	Double knockout	Normal	Up	Weber et al., 2006
PI3K 1/2/3/4/5/6	Sextuple knockout	Down	Up	Peracino et al., 2010
PI3K 1/2/3/4/5/6/PTEN	Septuple knockout	Down	Up	Peracino et al., 2010
PTEN	Knockout	Down	Normal	Peracino et al., 2010
RpkA	Knockout	Normal	Up	Riyahi et al., 2011
Sey1	Overexpression (WT)	n.t.	Up	Steiner et al., 2017a
	Overexpression (DN)	n.t.	Down
TBC1D5	Knockout	n.t.	Down	Bärlocher et al., 2017a
Villidin	Knockout	Down	Down	Fajardo et al., 2004

L. pneumophila uptake and intracellular growth in D. discoideum mutants was assessed by different methods. Effects (“up” or “down”) on bacterial uptake or intracellular growth are indicated relative to the parental amoeba strain or untreated amoebae, respectively. ATG, autophagy; PLC, phospholipase C; PI3K, phosphoinositide 3-kinase class I; PTEN, phosphatase and tensin homolog; Dd5P4, D. discoideum inositol 5-phosphatase 4; OCRL, oculocerebrorenal syndrome of Lowe; RpkA, receptor phosphatidylinositol kinase A; DN, dominant negative; WT, wild-type; n.t.: not tested.