Table 2.
Structure and physicochemical properties of the cationic peptides used in this study.
| Alternate name | Sequence | RP-HPLC | MALDI-TOF (M/Z) [M+H]+ | bNet charge | Residues | Hydro-phobic amino acids (%) | bGRAVY | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| atR(Min) | Theor. | Exper. | ||||||||||||||||||||
| Motif | 20R | R | W | Q | W | R25 | 4.33 | 985.55 | 986.66 | +3 | 6 | 33.3 | −3.133 | |||||||||
| Lineal/palindromic | R | W | Q | W | R | W | Q | W | R | 5.95 | 1,485.75 | 1,488.58 | +3 | 9 | 44.4 | −2.678 | ||||||
| Lfc B reference Peptide | 17F | K | C | R | R | W | Q | W | R | M | K | K | L | G | A31 | 5.25 | 1,992.09 | 1,994.71 | +6 | 15 | 33.3 | −1.207 |
| Dimeric | (R | R | W | Q | W | R)2 | K | Ahx | 5.21 | 2,195.24 | 2,198.51 | +6 | 15 | 26.7 | – | |||||||
| Tetrameric | (R | R | W | Q | W | R)4 | K2 | Ahx2 | C2 | 19.11 | 2,298.32c | 2,302.96c | +12 | 30 | 26.7 | – | ||||||
a
tR: Retention time of the main product (in minutes).
b
Net charge values and Grand Average of Hydropathy (GRAVY) values were calculated using the Antimicrobial Peptide Calculator and Predictor (http://aps.unmc.edu/AP/prediction/prediction_main.php). However, this was not possible for the branched peptides.
c
Experimental molecular weight that correspond to dimeric molecule before oxidation.