Table 5.
Antibacterial activity of the RRWQWR-based peptides against the clinical isolates of HCAI-relevant bacteria.
| Gram classification | Bacteria | Isolates | MIC50 (μM) | MBC (μM) | Resistant phenotype | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| LfcinB (20–25) | PLS | LfcinB (20–25)2 | LfcinB (20–25)4 | LfcinB (20–25) | PLS | LfcinB (20–25)2 | LfcinB (20–25)4 | ||||
| Monomer | Palindromic | Dimeric | Tetrameric | Monomer | Palindromic | Dimeric | Tetrameric | ||||
| Gram-positive | Enterococcus faecium | 550 | >100 | >100 | >100 | >100 | >100 | 100.0 | >100 | 12.5 | STR, CIP, LVX, ERY, TEC, VAN, TET, SXT |
| 1,040 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | STR, CIP, LVX, ERY, TEC, VAN, TET, TMS | ||
| 1,225 | >100 | >100 | >100 | >100 | >100 | 100.0 | >100 | 12.5 | STR, CIP, LVX, ERY, TEC, VAN, TET, TMS | ||
| 1,461 | >100 | >100 | >100 | >100 | >100 | 100.0 | >100 | 12.5 | STR, CIP, LVX, ERY, TEC, VAN, TET, TMS | ||
| 1,462 | >100 | 5.7 | 49.8 | >100 | >100 | 100.0 | >100 | 12.5 | STR, CIP, LVX, ERY, TEC, VAN, TET y TMS | ||
| Staphylococcus aureus | 52,013 | 5.1 | 8.3 | 12.6 | 6.7 | >100 | 100.0 | 25.0 | 12.5 | Sensitive | |
| 43,062 | 5.1 | 50.4 | 13.5 | 6.7 | >100 | 100.0 | 50.0 | 12.5 | OXA | ||
| 43,337 | 3.7 | 13.0 | 13.5 | 6.3 | >100 | 50.0 | 25.0 | 12.5 | OXA | ||
| 48,575 | 3.7 | 36.5 | 13.4 | 9.2 | >100 | 100.0 | 25.0 | 12.5 | CLI | ||
| 48,577 | 3.7 | 5.7 | 12.9 | 6.3 | >100 | 100.0 | 50.0 | 100.0 | OXA | ||
| Gram-negative | Klebsiella pneumoniae | 49,644 | >100 | 12.5 | 12.3 | ND | >100 | 100.0 | 50.0 | ND | IPM, MEM, ETP, DOR, FEP, CAZ, CRO, TZP, CIP |
| 50,181 | >100 | >100 | 6.6 | 5.5 | >100 | >100 | 100.0 | 100.0 | IPM, MEM, ETP, DOR, FEP, CAZ, CRO, TZP | ||
| 50,424 | 11.3 | 5.6 | 5.8 | 7.8 | >100 | >100 | >100 | 100.0 | IPM, MEM, ETP, DOR, FEP, CAZ, CRO, TZP, GEN, CIP | ||
| 51,048 | >100 | 11.7 | 5.7 | 5.2 | >100 | >100 | 100.0 | 50.0 | IPM, MEM, ETP, DOR, FEP, CAZ, CRO, TZP | ||
| 51,009 | >100 | 25.6 | 5.6 | 1.6 | >100 | 100.0 | 25.0 | 25.0 | MEM, ETP, CTX, CRO, AMK, GEN, SXT, FEP, CAZ y CIP, NOR, NIT | ||
| Pseudomonasss aeruginosa | 47,661 | >100 | 75.0 | 13.4 | 7.5 | >100 | >100 | >100 | 25.0 | MEM, IPM, FEP, CAZ, CIP, AMK, GEN, TGC | |
| 48,220 | >100 | >100 | 50.0 | 12.6 | >100 | >100 | 50.0 | 25.0 | Sensitive | ||
| 48,221 | >100 | >100 | 64.2 | 11.9 | >100 | >100 | >100 | 50.0 | MEM, FEP, CAZ, AMK, CIP | ||
| 48,458 | >100 | >100 | 75.0 | 72.8 | >100 | >100 | >100 | >100 | MEM, GEN, CAZ, FEP, AMK | ||
| 48,526 | >100 | >100 | 36.3 | 24.9 | >100 | 100.0 | >100 | >100 | Sensitive | ||
Amikacin (AMK), cefepime (FEP), cefotaxime (CTX), ceftriaxone (CRO), ceftazidime (CAZ), ciprofloxacin (CIP), clindamycin (CLI), doripenem (DOR), ertapenem (ETP), erythromycin (ERY), gentamicin (GEN), imipenem (IPM), levofloxacin (LVX), meropenem (MEM), oxacillin (OXA), piperacillin-tazobactam (TZP), streptomycin (STR), teicoplanin (TEC), tetracycline (TET), tigecycline (TGC), trimethoprim-sulfamethoxazole (SXT), vancomycin (VAN).
ND: Not determined.