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. 2018 Mar 6;9:967. doi: 10.1038/s41467-018-03433-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — EDC4 interacts and with the BRCA1-BRIP1-TOPBP1 complex. a Diagram showing the region of TOPBP1 used as bait for the Y2H screen and the different cDNAs from EDC4 captured. b Immunoblots showing that endogenous EDC4 interacts with TOPBP1 in HeLa cells. EDC4 was immunoprecipitated and analyzed by immunoblotting with indicated antibodies. c Immunoblots showing that exogenous EDC4 interacts with exogenous TOPBP1 in HeLa cells. EDC4 was immunoprecipitated from HeLa cells expressing both tagged EDC4 and TOPBP1 and analyzed by immunoblotting with indicated antibodies. d Immunoblots showing that exogenous TOPBP1 interacts with exogenous EDC4 in HeLa cells. TOPBP1 was immunoprecipitated from HeLa cells expressing both tagged EDC4 and TOPBP1 and analyzed by immunoblotting with indicated antibodies. e Cellular fractionation of HeLa cells shows that EDC4 is not only a cytoplasmatic protein but also present at the nucleus and the chromatin. f Immunoblots showing that endogenous EDC4 interacts with BRCA1 and BRIP1 in HeLa cells. BRCA1 was immunoprecipitated from HeLa cells and analyzed by immunoblotting with indicated antibodies