Table 3.
Characteristic ions of standard sterols in full scan mode by LC-MS/MS.
| Compound | RT (min) | M | M-H2O+H+ | Specific ions for identification Ion [M-H2O+H+] > Ions from spectrum |
|---|---|---|---|---|
| Ergosterol | 3.2 | 396 | 379 | 379 > 158.9; 184.9; 199; 213; 225; 239; 253; 295; 309; 323 |
| Brassicasterol | 3.9 | 398 | 381 | 381 > 201.3; 203.3; 215.2; 217.3; 241.2; 255.3; 257.4; 271.1; 297.3; 299.3 |
| Stigmasterol | 4.9 | 412 | 395 | 395 > 255; 297; 283; 311; 241; 201 |
| Campesterol | 4.9 | 400 | 383 | 383 > 147; 149; 161; 175; 189; 203; 215; 229; 243; 257 |
| β-Sitosterol | 5.7 | 414 | 397 | 397 > 160.9; 174.9; 188.9; 202.9; 214.9; 243; 257; 287.1; 315.2 |