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. 2018 Feb 5;7(1):11–31.

Figure 2.

Figure 2

The rough-eye morphology induced by TBPH knockdown is modified by genetic changes in ter94. Each panel shows a scanning electron micrograph of the adult compound eye. Each lower panel shows a higher magnification image of the corresponding upper panel. (A) GMR-GAL4/Y; UAS-GFP-IR/+; + (GMR>UAS-GFP-IR/+). The eyes of control flies exhibited apparently normal eye morphology including an organized ommatidial architecture. (B) GMR-GAL4/Y; UAS-TBPH-IR81-181/+; + (GMR>UAS-TBPH-IR81-181/+); (C) GMR-GAL4/Y; UAS-TBPH-IR517-531/+; + (GMR>UAS-TBPH-IR517-531/+). Adult eyes from two independent fly lines with eye-specific TBPH knockdown. Flies carrying GMR>UAS-TBPH-IR81-181/+ (B) or GMR>UAS-TBPH-IR517-531/+ (C) had essentially the same rough-eye morphology and exhibited ommatidial degeneration. (D) GMR-GAL4/Y; UAS-TBPH-IR517-531/ter94K15502; + (GMR>UAS-TBPH-IR517-531/ter94K15502), (E) GMR-GAL4/Y; UAS-TBPH-IR517-531/ter9403775; + (GMR>UAS-TBPH-IR517-531/ter9403775). Each of the flies carrying GMR>UAS-TBPH-IR517-531/ter94K15502 (D) and GMR>UAS-TBPH-IR517-531/ter9403775 (E) showed rough-eye morphology that was enhanced relative to that observed in flies with GMR>UAS-TBPH-IR517-531/+ alone (C). (F) GMR-GAL4/Y; UAS-TBPH-IR517-531/UAS-GFP; + (GMR>UAS-TBPH-IR517-531/UAS-GFP); (G) GMR-GAL4/Y; UAS-TBPH-IR517-531/UAS-ter94; + (GMR>UAS-TBPH-IR517-531/UAS-ter94). The flies carrying GMR>UAS-TBPH-IR517-531/UAS-ter94 (G) suppressed rough-eye morphology that observed in flies with GMR>UAS-TBPH-IR517-531/UAS-GFP (F). Posterior is to the right, and dorsal is to the top. The flies developed at 28°C. Scale bars in each upper panel indicate 50 µm and in the bottom panel indicate 14.2 µm. The rough areas of the compound eyes are marked with dotted lines.