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. 2018 Mar 6;8:4049. doi: 10.1038/s41598-018-21371-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Images from time-lapse video demonstrates fusion of MATR3:YFPΔRRM2 spheres in C2C12 cells. An example of the timing of fusion is illustrated in this panel of images. Two independent fusion events can clearly be seen to occur over a period of 15–30 minutes in each case. The example shown is representative of observations made in 3 separate transfection experiments tracking 20–30 cells in each field of view. Original magnification 20X.