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Detection of IR/A and IR/B isoforms in cultures of human neurons and microglia cells by qPCR. Total RNA from differentiated SH-SY5Y neurons and human primary human microglia-SV40 cells were extracted and assayed by qPCR with the insulin receptor primers described in Table 1 and human ß actin internal control primers. Results are expressed as 1/ΔΔCt for (A) IR/A and (B) IR/B. (C) The ratio of IR/A:IR/B was determined from the results of the qPCR. *p < 0.05 compared to neurons.
