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. 2018 Mar 6;8:4070. doi: 10.1038/s41598-018-22434-2

Figure 3.

Figure 3

Detection of IR/A and IR/B isoforms in vitro in human neurons and microglia cells by in situ RT-PCR/FISH. (A) SH-SY5Y neuronal cells were differentiated for 7 days on glass coverslips and assayed for (B) IR/A (green) and (C) IR/B (red). Coverslips were mounted with Vectashield containing DAPI to visualize the nuclei. Scale bar = 20 µm. (D) SV40 human microglia cells were grown on glass coverslips and assayed for (E) IR/A (green) and (F) IR/B (red) and then mounted with Vectashield containing DAPI (blue) to visualize the nuclei. Scale bar = 10 µm.