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Expression of early progenitor markers within a representative clonal CPC population. CPCs used in experiments were screened for the co-expression of Isl1 and c-kit (A), as indicated by flow cytometry. These Isl1+c-kit+ CPCs also exhibit other markers of early cardiac development, including KDR and PDGFRA, as well as the chemokine receptor CXCR4 (B). Dotted lines indicate isotype controls and solid lines indicate labeled CPCs. Double-labeled cells are shown as a dot-plot.
