Figure 2.

Short-term hypoxia upregulates Akt pathway activity in neonatal CPCs. Changes to gene expression following exposure to short-term hypoxia were analyzed using Ingenuity Pathway Analysis (A). The bar chart represents significance of gene enrichment, (i.e., -log (p-value) for that pathway) while the yellow line indicates the ratio of genes from the dataset that map to the pathway and the total number of genes in that pathway. Pathway analysis indicated that the PI3K/AKT pathway (B) was among the most statistically affected pathways. RT-PCR experiments (C) confirmed that hypoxia preconditioning resulted in a significant increase in expression of Akt-related genes (n=9). Akt phosphorylation was then measured using flow cytometry (D, E). Representative histogram (D) of the measured increase in P-Akt (E; n=3). Western blot analysis was used to confirm the increase in Akt phosphorylation (F) from normoxia (N) to hypoxia (H). Positive (+) and negative (-) controls are shown. *P < 0.05. Values reported as the average ± S.E.M.