Figure 3.

Short-term hypoxia upregulates Akt pathway activity in adult CPCs. RT-PCR experiments (A) demonstrated that hypoxia preconditioning resulted in a significant increase in expression of Akt-related genes in adult CPCs (n=5). Akt phosphorylation was then measured using flow cytometry (B; n=3). Western blot was used to confirm the increase in P-Akt (C; n=3) from normoxia (N) to hypoxia (H). Positive (+) and negative (-) controls are shown. (D) Adult CPCs were treated with SDF-1α alone or in combination with hypoxia. P-AKT expression was resolved using western blot, and fold changes in phosphorylated Akt, relative to β-actin, were quantified using ImageJ. *P < 0.05. Values reported as the average ± S.E.M.