Figure 5.

Cell survival improves following CPC treatment with short-term hypoxia. Expression of genes associated with cell survival increased after hypoxia pre conditioning in both neonatal (A; n=3) and adult (B; n=3) CPCs. To assess cell survival, apoptosis was induced using 0.5 mM H₂O₂ in both control and hypoxia-treated CPCs and relative DNA fragmentation was measured using Brd-U. Flow cytometry (C) of BrdU-DNA binding within a representative clone, with and without hypoxic pretreatment, is shown. CPCs treated with short-term hypoxia exhibited an approximate 50% decrease in apoptotic cells after induction of cellular death by 0.5 mM H₂O₂. Quantification of TUNEL assay results obtained using three neonatal (D) and three adult (E) adult clones is shown. Apoptosis in response to oxidative stress trends downward in the hypoxia-pre-conditioned group. *P < 0.05, **P < 0.01. Values reported as the average ± S.E.M.