Table 2.
Yield of recombinant salmocins expressed in Nicotiana benthamiana plants.
| No. | Bacteriocin | Harvest (dpi) | Yield (mg/g FW)* | Yield (% TSP)* | ||||
|---|---|---|---|---|---|---|---|---|
| AV | SD | N | AV | SD | N | |||
| 1 | SalE2 | 6 | 1.7 | 0.2 | 3 | 25.0 | 0.0 | 3 |
| 2 | SalE3 | 5 | 1.6 | 0.2 | 3 | 37.0 | 10.4 | 3 |
| 3 | SalE7 | 5 | 1.4 | 0.3 | 3 | 18.0 | 6.9 | 3 |
| 4 | SalE1a | 5 | 1.2 | 0.2 | 3 | 20.3 | 3.1 | 3 |
| 5 | SalE1b | 4 | 1.2 | 0.1 | 3 | 25.7 | 3.1 | 3 |
*The yield was calculated in mg/g fresh weight of plant biomass and as a percentage of TSP (based on extraction with 50 mM HEPES pH 7.0, 10 mM K acetate, 5 mM Mg acetate, 10% (v/v) glycerol, 0.05% (v/v) Tween-20, 300 mM NaCl and syringe inoculation of plant material with 1:100 dilutions of agrobacterial cultures (OD600 = 1.3)) and is represented as an average value and standard deviation (AV, SD) of several experiments. N, number of independent experiments. In each experiment, plant material pooled from 3 plants was analyzed for each protein.