Figure 2.

Growth and LG utilization of the isolate thermophiles in LG medium. (a) Each isolate was inoculated into 50 ml of LG medium as the OD₆₆₀ of the medium was 0.02. The cultivation was carried out at 50°C for S-2701M and 60°C for S-1501M and S-65801 with reciprocal shaking at 100 rpm. Circle, square, and triangle symbols represent the growth of S-1501M, S-2701M, and S-65801, respectively. (b) Culture medium was withdrawn at the time indicated in the figure and centrifuged. Ten microliters of the supernatant was spotted on to a silica gel plate and developed with a solvent system composed of n-butanol/acetic acid/water (4:5:1). The spots were detected by anisaldehyde solution. One milligram per milliliter of glucose and LG were used as standards.