Skip to main content
. 2018 Mar 6;8:4048. doi: 10.1038/s41598-018-22440-4

Figure 3.

Figure 3

Internalisation of Ecrg4(71–132) and activation of NF-κB by Ecrg4(71–132) via several scavenger receptors. (A) HEK293 T cells were transfected with several Flag-tagged scavenger receptors. Two days after transfection, the cells were treated with Ecrg4(71–132) for 2 h. Ecrg4(71–132)–Fc was detected with a FITC-labelled anti-human IgG Fc antibody (green) and the transiently expressed Flag-tagged receptors were detected with an anti-Flag antibody (red). Nuclei were stained with Hoechst (blue). (B) Fluorescence intensities of Ecrg4(71–132) bound to primary microglia. Primary microglia were incubated with Fc(N293A)-fused Ecrg4(71–132) for 1 h in the presence of PBS, polyI, or polyC. (C) Immunoblotting for p65 in microglial cells treated with Ecrg4(71–132) in the presence of PBS, polyI, or polyC. The band intensity was quantified by a ChemiDoc™ MP Imaging system (Bio-Rad), and the relative band intensity ratio NF-κB p65(pSer536)/NF-κB p65 is shown (pSer536/cont). Representative data from three independent experiments are indicated.