Fig. 6. SESN1 inhibits myoblast apoptosis via the p53 signaling pathway.

a, c The mRNA expression levels of several apoptosis-related genes with SESN1 overexpression and knockdown in CPMs. b, d Western blotting analysis of several cleaved caspases after SESN1 overexpression and inhibition in CPMs. The numbers shown below bands were folds of band intensities relative to control. Band intensities were quantified by ImageJ and normalized to GAPDH. Data are expressed as a fold change relative to the control. e–h Both CPMs and QM-7 cells were collected for the detection of apoptotic cells by flow cytometry, 48 h after transfection. In all panels, data are presented as means ± S.E.M. of three independent assays. Statistical significance of differences between means was assessed using an unpaired Student’s t-test (*P < 0.05; **P < 0.01) vs. NC, negative control