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. 2018 Mar 6;8:4069. doi: 10.1038/s41598-018-22447-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Replication kinetics of parental and mutant viruses. Confluent DEF cells monolayers were infected with each virus shown at an MOI of 0.01. Viral titer of infected supernatant, cells and mixture of cells cultures were determined at the indicated time points by measuring TCID₅₀ on DEF cells. All titrations were carried out in three independent experiment. The titers obtained were averaged, and the standard error of the mean was calculated each time point.