Figure 3.

The multivariate concentric square field (MCSF) test arena with the defined zones labeled. Animals were placed in the center zone at the start position and were allowed to explore the arena for 20 min. Arrows show the different paths that the animals could take. Corr A–C = Corridors A–C (a). The distance moved in the MCSF arena decreased slightly over time, for all age‐groups regardless of genotype (b). The distance moved (cm) and the velocity (cm/s) in the MCSF arena were significantly lower in A30P α‐syn tg animals compared to control animals at 8 months (c, d). The number of rearings (wall rearing and free rearing combined) was increased in 8‐ and 11‐month‐old A30P α‐syn tg mice compared to wt age‐matched controls (e). The A30P α‐syn tg mice entered less zones compared to controls at 8 months of age (e). The risk/shelter index was increased in the A30P α‐syn tg mice as compared to control mice at 8 months of age (g). Representative images of tracks in the MCSF test show that the A30P α‐syn tg mice displayed a reduced general activity, but crossed the illuminated bridge area more compared to control animals (H). Values are expressed as mean ± SD, *p < .05, **p < .01, ***p < .001