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. 2018 Mar;188(3):768–784. doi: 10.1016/j.ajpath.2017.10.022

Figure 3.

Figure 3

Metanephric mesenchymal (MM) cell expression of RNA encoding mesenchymal and endothelial markers by RT-PCR. Total RNA was extracted from MM and ureteric bud (UB) cell lysates and reverse transcribed to synthesize cDNA with the use of the SYBR Green method. Ratio of cDNA transcript of marker: glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is shown for each cell line. The results show high transcript levels of mesenchymal markers forkhead box D1 (Foxd1), glioma-associated oncogene homolog 1 (Gli-1), and ANG-1 (A–C), as well as endothelial markers aquaporin 1 (AQP1), Flt-1 (vascular endothelial growth factor receptor 1; VEGFR1), Tie-2 (angiopoietin receptor), and platelet endothelial cell adhesion molecule (PECAM) (D–G) in MM cells relative to UB cells. Data are expressed as means ± SEM. n = 3 independent experiments. ∗P < 0.05 versus UB cells. Ang-1, angiopoietin 1.