Table 1.
Differentiation Markers: Antibody Source, Targets, Species, and Concentrations
| Primary antibody | Target cell | Source | Species/concentration |
|---|---|---|---|
| Mesenchymal markers | |||
| α-SMA (1A4) | Mesenchymal, pericytes | Sigma-Aldrich, St. Louis, MO | Mouse/10 μg/mL |
| Vimentin (V13.2) | Mesenchymal, pericytes | Sigma-Aldrich | Mouse/10 μg/mL |
| PDGFR-β (958) | Mesenchymal, pericytes | Santa Cruz Biotechnology, Santa Cruz, CA | Rabbit/10 μg/mL |
| Foxd1 (C-19) | Mesenchymal, stromal, endothelial | Santa Cruz Biotechnology | Goat/20 μg/mL |
| Gli1 (H-300) | Pericytes, endothelial | Santa Cruz Biotechnology | Rabbit/10 μg/mL |
| Endothelial markers | |||
| Aquaporin 1 (H-55) | Endothelial | Santa Cruz Biotechnology, Santa Cruz, CA | Rabbit/10 μg/mL |
| Flk-1 (C-20) | Endothelial | Santa Cruz Biotechnology | Rabbit/10 μg/mL |
| Flt-1 (H-225) | Endothelial | Santa Cruz Biotechnology | Rabbit/10 μg/mL |
| Tie-2 (C-20) | Endothelial | Santa Cruz Biotechnology | Rabbit/20 μg/mL |
| PECAM (CD-31) | Endothelial | Abcam, Cambridge, MA | Rabbit/10 μg/mL |
α-SMA, α-smooth muscle actin; Flk-1, vascular endothelial growth factor receptor 2; Flt-1, vascular endothelial growth factor receptor 1; Foxd-1, forkhead box D1; Gli-1, glioma-associated oncogene homolog 1; PDGFR, platelet-derived growth factor receptor; PECAM, platelet endothelial cell adhesion molecule.