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. 2018 Mar;188(3):768–784. doi: 10.1016/j.ajpath.2017.10.022

Table 2.

RT-PCR Primers for Mice Vasculogenesis Markers

Gene Forward primer Reverse primer Ref
AQP1 5′-CTCCCTAGTCGACAATTCAC-3′ 5′-ACAGTACCAGCTGCAGAGTG-3′ Lanaspa et al20
FOXD1 5′-ACTCAGCCCTGGCCTTGC-3′ 5′-GCGGGAGGAGTGAGAATCTAGAA-3′ Carreres et al21
GLI1 5′-GCAGTGGGTAACATGAGTGTCT-3′ 5′-AGGCACTAGAGTTGAGGAATTTGT-3′ Mathew et al22
PDGFRB 5′-TGCCTCAGCCAAATGTCACC-3′ 5′-TGCTCACCACCTCGTATTCC-3′ Shi et al23
ANGPT1 5′-CATTCTTCGCTGCCATTCTG-3′ 5′-GCACATTGCCCATGTTGAATC-3′ Shi et al23
FLK1 5′-GCCCTGCTGTGGTCTCACTAC-3′ 5′-CAAAGCATTGCCCATTCGAT-3′ Shi et al23
FLT1 5′-GAGGAGGATGAGGGTGTCTATAGGT-3′ 5′-GTGATCAGCTCCAGGTTTGACTT-3′ Shi et al23
TIE2 5′-ATGTGGAAGTCGAGAGGCGAT-3′ 5′-CGAATAGCCATCCACTATTGTCC-3′ Shi et al23
PECAM 5′-GAGCCCAATCACGTTTCAGTTT-3′ 5′-TCCTTCCTGCTTCTTGCTAGCT-3′ Shi et al23
CDH5 5′-TCCTCTGCATCCTCACTATCACA-3′ 5′-GTAAGTGACCAACTGCTCGTGAAT-3′ Shi et al23
PDGFB 5′-TGAAATGCTGAGCGACCAC-3′ 5′-AGCTTTCCAACTCGACTCC-3′ Shi et al23
VEGFA 5′-GGAGATCCTTCGAGGAGCACTT-3′ 5′-GGCGATTTAGCAGCAGATATAAGAA-3′ Shi et al23
GAPDH 5′-GCAAAGTGGAGATTGTTGCCAT-3′ 5′-CCTTGACTGTGCCGTTGAATTT-3′ Shi et al23