Table 1.
Summary of miRNA Expression Profiling Studies in Preinvasive Breast Cancer
| Sample type | Analysis method | Major findings | miRNA expression∗ |
Reference | |||||
|---|---|---|---|---|---|---|---|---|---|
| Normal-ADH | Normal-DCIS | DCIS-IBC | |||||||
| Microdissected epithelial cells: RM (n = 9), paired normal (n = 8) and DCIS (n = 8) | RT-qPCR, TaqMan low-density array | 99 miRNAs were differentially expressed between DCIS and normal mammary epithelial cells | ↑miR-7 ↑miR-18a ↑miR-21 ↑miR-93 ↑miR-99b ↑mir-181b ↑miR-182 ↑miR-183 ↑miR-191 ↑miR-193b ↑miR-200b/c ↑miR-324-5p ↑miR-365 ↑miR-425-5p ↑miR-449b |
↓let-7c ↓miR-10b ↓miR-99a ↓mir-125b ↓miR-127 ↓miR-130a ↓miR-145 ↓miR-195 ↓miR-204 ↓miR-376a ↓miR-382 ↓miR-410 ↓miR-511 |
Hannafon et al5 | ||||
| Bulk extracted tissues: normal (n = 11), DCIS (n = 17), IBC (n = 151) | Solexa (Cambridge, UK) next-generation sequencing (in house) | Normal breast tissues may be distinguished from DCIS and IBC by increased miR-21 and decreased miR-98 and let-7 levels; most changes in miRNA expression already present in DCIS | ↑miR-21 ↑miR-142-3p ↑miR-142-5p |
↓miR-22 ↓miR-98 cluster (miR-125a, miR-99a, let-7a) ↓miR-451 ↓miR-144 ↓miR-143* ↓miR-320 ↓miR-378 ↓miR-497 |
↑miR-142-3p | ↓miR-125a ↓miR-451 ↓miR-144 ↓miR-145 ↓miR-143 ↓miR-378 |
Farazi et al24 | ||
| Subset of samples from Farazi et al, 201124: normal (n = 6), DCIS (n = 8), IBC (n = 80) | Reanalysis of Solexa next-generation sequencing-used only the most reliable results | 66 miRNAs were deregulated in the normal-DCIS† transition, whereas only nine were different between the DCIS-IBC transition; most changes in miRNA expression already present in DCIS | ↑miR-21 ↑miR-200c ↑miR-16 ↑miR-142-5p/3p ↑miR-374a ↑miR-26b ↑miR-29b ↑miR-183 ↑miR-96 ↑miR-106b ↑miR-182 ↑miR-361-5p |
↓miR-320 ↓miR-378 ↓miR-127-3p ↓let-7b, c, d ↓miR-376a/c ↓miR-193a/b ↓miR-99a ↓miR-22 ↓miR-423-5p ↓miR-145 ↓miR-125b ↓miR-497 |
↑let-7d ↑miR-181a ↑miR-210 ↑miR-221 |
↓miR-10b ↓miR-126 ↓miR-143 ↓miR-218 ↓miR-335-5p |
Volinia et al25 | ||
| Laser capture–microdissected FFPE tissues: normal (n = 8), ADH (n = 4), DCIS (n = 6), IBC (n = 7) | miRNA microarray | ↑miR-21 ↑miR-200b ↑miR-15b ↑miR-183 ↑miR-30d |
↓miR-1275 ↓miR-638 ↓miR-572 ↓miR-671-5p |
↑miR-556-3p | ↓miR-557 ↓miR-1207-5p ↓miR-874 |
Chen et al26 | |||
| Bulk-extracted fresh-frozen tissues: normal (n = 186), DCIS (n = 18), IBC (n = 1338) | miRNA microarray | 70 miRNAs were deregulated from normal-to-DCIS tissues; no miRNAs were deregulated in DCIS-to-IBC transition; breast cancer subtype–specific miRNA expression patterns were observed | ↑miR-21-5p ↑miR-96-5p ↑miR-106b-5p ↑miR-142-5p/3p ↑miR-155-5p ↑miR-183-5p ↑miR-200b/c-3p ↑miR-342-3p ↑miR-425-5p ↑miR-429-3p |
↓let-7b/c-5p ↓miR-22-3p ↓miR-99a-5p ↓miR-100-5p ↓miR-125b-5p ↓miR-140-3p ↓miR-143-5p ↓miR-145-5p/3p ↓miR-193a-5p ↓miR-193b-3p ↓miR-378a-3p ↓miR-497-5p ↓miR-652-3p |
↑miR-106b-5p ↑miR-142 ↑miR-342-3p ↑miR-425-5p |
↓let-7c-5p ↓miR-125b-5p ↓miR-140-3p ↓miR-145-5p/3p ↓miR-193a-5p ↓miR-378a-3p |
Haakensen et al27 | ||
↑, Increased; ↓, decreased; ADH, atypical ductal hyperplasia; DCIS, ductal carcinoma in situ; FFPE, formalin-fixed, paraffin-embedded; IBC, invasive breast cancer; RM, reduction mammoplasty; RT-qPCR, quantitative RT-PCR.
∗
Fold change ≥1.5.
†
Top 12 differentially expressed miRNAs are listed.