Figure 4.
Effect of Cby overexpression on the cell cycle distributions and apoptosis of SUNE1 cells. (A) Cell cycle analysis was performed by flow cytometry following plasmid transfection. The proportion of G1 phase cells for plv-cs2.0 (empty vector) and plv-cs2.0-Cby groups were 52.5±2.5 and 73.6±3.2%, respectively (P=0.0051). (B) Apoptosis analysis of the control group plv-cs2.0 and plv-cs2.0-Cby SUNE1 cells. Hoechst 33342 staining showed apoptotic cells with condensed chromatin or fragmented nuclei. Magnification, ×200. (C) Assessment of apoptosis of SUNE1 cells using flow cytometry with Annexin V-fluorescein isothiocyanate/phosphoinositide staining following treatment with 20 µM 5-fluorouracil (left) or 30 nM Wnt 3a (right). *P<0.05, **P<0.01. Cby, Chibby.