Figure 3.

Percentage of CD8a⁺ T cells in circulating peripheral blood monocytes prior to therapeutic xenovaccination (day 16, white column), 3 days after the completion of xenovaccination (day 26, black columns) and at the end of the study (day 70, grey columns). There were no significant changes in the levels of circulating CD8a⁺ T cells 3 days after the completion of xenovaccination, compared with the levels prior to the start of vaccination (within and between the experimental arms). However, on day 70 (at the end of the observation period), the levels of circulating CD8a⁺ T cells were significantly higher in the surviving mice vaccinated with the xeno chicken vaccine (n=10) compared with surviving mice (n=4) in the control group (P=0.002) or in the Xeno Rat group (P=0.049). In xeno chicken vaccine-treated mice, the level of CD8a⁺ T cells on day 70 was significantly higher compared with the level on day 16 (following tumor removal, prior to xenovaccination) (P=0.036) and on day 26 (3 days after the completion of xenovaccination) (P=0.003). There were no significant differences in the levels of circulating CD8a⁺ T cells in the mice that were treated with xeno rat vaccines evaluated at different time points or comparing T-cell levels with those in control group animals. Additionally, there was a significantly lower CD8a⁺ T-cell population in the control mice at the end of the study (day 70) compared with the level following the completion of vaccination (day 16) (P=0.028). CD8a, cluster of differentiation 8a. *P≤0.05.