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. 2018 Jan 17;10(3):e8047. doi: 10.15252/emmm.201708047

Figure 6. GPR120 is involved in differentiation of brown adipocytes and browning of white adipocytes.

Figure 6

  1. Immortalized murine brown adipocytes (n = 3) were differentiated for 0, 2, 4, 6, or 7 days after which expression of Gpr120 and Ucp1 was determined by qRT–PCR. On day 7, a subset of adipocytes (n = 3) was stimulated with CL (10 μM) or vehicle.
  2. Expression of Gpr120 and Ucp1 was measured in undifferentiated (Undiff), differentiated (Diff), and CL‐treated (Diff + CL) brown adipocytes (BA), subcutaneous white adipocytes (sWA), and sWA treated with the browning agent rosiglitazone (sWA brite) (n = 3).
  3. WT and GPR120 KO brown adipocytes (n = 3) were treated with vehicle or TUG‐891 (10 μM) throughout differentiation and stained at day 0, 3, 7, and 9 of differentiation with Oil Red O. Absorbance of the staining at 520 nm was quantified. A representative image at day 8 of differentiation was taken with a phase‐contrast microscope (Leica) at 20‐fold magnification.
  4. As in (C), WT and GPR120 KO brown adipocytes (n = 3) were treated with vehicle or TUG‐891 throughout differentiation to analyze expression patterns of aP2 and Ucp1.
Data information: Data represent means ± SEM. **P < 0.01 compared to the vehicle group, ***P < 0.001 compared to the WT control group or indicated controls, according to the two‐tailed unpaired Student's t‐test (A) or two‐way ANOVA with Dunnett's post hoc test (B–D). The exact P‐value for each significant difference can be found in Appendix Table S5.