Figure 1.

S100A6 promotes the proliferation of cervical cancer cells (A) qPCR was used to analyze the mRNA levels of S100A6 in the cervical cancer cell lines HeLa, SiHa and CaSki. (B) AdS100A6 infected HeLa and CaSki cells, AdsiS100A6 infected CaSki and SiHa cells. The infection efficiency was determined by western blotting. Cell proliferation was detected using an MTT assay in (C) HeLa and CaSki cells following treatment with AdS100A6 and in (D) SiHa and CaSki cells following treatment with AdsiS100A6. Cell apoptosis was determined by Hoechst staining assay in (E) HeLa and CaSki cells after treatment with AdS100A6 and in (F) SiHa and CaSki cells following treatment with AdsiS100A6. As no significant changes were identified between all groups in number of apoptosis cells, no quantitative data was provided. *P<0.05, **P<0.01, ***P<0.005 compared with the AdGFP or AdRFP groups. All the experiments were repeated three times. Results were presented as the mean ± standard deviation. A one-way analysis of variance, followed by the Student-Newman-Keuls test was used to analyze the statistical significance of differences among groups. OD, optical density; nm, nanometer; AdGFP, adenovirus carrying green fluorescent protein; AdS100A6, adenovirus carrying S100A6; AdRFP, adenovirus carrying red fluorescent protein; AdsiS100A6, adenovirus carrying S100A6-short interfering RNA gene; E-cadherin, epithelial cadherin; N-cadherin, neuronal cadherin.