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Akt phosphorylation is involved in Rap2c-mediated cancer cell migration and invasion. (A) Western blot analysis of the indicated protein levels in U2OS cells overexpressing Rap2c or with Rap2c knockdown. (B) Densitometry analysis of p-Akt473. *P<0.05 vs. si-Ctrl, **P<0.01 vs. Vector. Rap2c, Ras-related protein Rap2c; p-Akt, phosphorylated protein kinase B; Ctrl, control; Timp2, tissue inhibitor of metalloproteinase 2; Bcl-2, B-cell lymphoma-2; Bax, Bcl-associated X.
