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. 2018 Feb 5;15(4):4900–4906. doi: 10.3892/ol.2018.7956

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Copyright: © Xie et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Fluorescence-activated cell sorting of SP and non-SP cells using flow cytometry. (A) Hoechst 33342^low/neg fraction accounted for 1.09% of live cells, which decreased to 0.2% after treatment with Verapamil, and the sorted purity of SP cells was 99%. (B) Hoechst 33342^low/neg and Hoechst 33342^high cells were identified in the SP fraction, with 52.86% of living cells being Hoechst 33342^low/neg. However, the percentage decreased to 0.31% following treatment with Verapamil. (C) Only Hoechst 33342^high cells were identified in the NSP fraction. Hoechst 33342^low/neg cells were isolated at a very low rate of 0.13% in living cells, which decreased further to 0.06% following treatment with Verapamil. SP, side population; NSP, non-side population.