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. 2018 Feb 1;15(4):4827–4836. doi: 10.3892/ol.2018.7922

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Copyright: © Fu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — NP-visnagin inhibited apoptosis in the ischemic region subsequent to IR. (A) In order to examine the effect of NP-visnagin on the rate of apoptosis in IR injury, a TUNEL assay was performed; green fluorescence indicates TUNEL-positive nuclei, indicating apoptotic cells, whereas blue fluorescence (DAPI) indicates all cells. (B) Quantification of apoptotic cells. (C) Caspase-3 and. (D) caspase-9 activity of the myocardium subsequent to IR. **P<0.01; ***P<0.005. NP, nanoparticles; IR, ischemia/reperfusion; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling.