Table 1. A list of primers used in this study.
| Gene | Primer Name | Sequence | Description |
|---|---|---|---|
| MDH1 | MDH1 KO pFA6 F | AAAAAAAACAAAAGGAAAAGGAAGGATACCATATACAATGCGGATCCCCGGGTTAATTAA | Primer for KO of gene using pFA6 plasmids. |
| MDH1 | MDH1 KO pFA6 R | TTCCCTATTTTTCACTCTATTTCTGATCTTGAACAATCTAGAATTCGAGCTCGTTTAAAC | Primer for KO of gene using pFA6 plasmids. |
| MDH1 | MDH1 N' tag CHK R | TGGAACGGTAGAATTGACTG | Primer for checking N' tagging of the gene. |
| MDH1 | MDH1 WT CHK F | TCCAACCCAGTCAATTCTAC | Primer for testing the presence of the gene. |
| MDH1 | MDH1 WT CHK R | GTTAGCGAATTTAGCACCAG | Primer for testing the presence of the gene. |
| MDH2 | MDH2 KO pFA6 F | CAAAAGTTCAATACAATATCATAAAAGTTATAGTAACATGCGGATCCCCGGGTTAATTAA | Primer for KO of gene using pFA6 plasmids. |
| MDH2 | MDH2 KO pFA6 R | CAATTTGCTGCATTCTTATGCTTCGGTCCGATGCTCATTAGAATTCGAGCTCGTTTAAAC | Primer for KO of gene using pFA6 plasmids. |
| MDH2 | MDH2 N' tag CHK R | TGGAAATGACAAGAACGAAG | Primer for checking N' tagging of the gene. |
| MDH2 | MDH2 WT CHK F | AACTGTTTGCATAACGCTTC | Primer for testing the presence of the gene. |
| MDH2 | MDH2 WT CHK R | CATGGAGTTAACACGAGGAG | Primer for testing the presence of the gene. |
| MDH2 | MDH2 C' tag CHK F | ATTCCGTTGTTTTCACAGTC | Primer for checking C' tagging of the gene. |
| MDH2 | MDH2 C' tag pFA6 F | TAAGGGCTTGGAATTCGTTGCATCGAGATCTGCATCATCTCGGATCCCCGGGTTAATTAA | Primer for C' tagging of gene using pFA6 plasmids. |
| MDH2 | MDH2 C' tag pFA6 R | CAATTTGCTGCATTCTTATGCTTCGGTCCGATGCTCATTAGAATTCGAGCTCGTTTAAAC | Primer for C' tagging of gene using pFA6 plasmids. |
| MDH3 | MDH3 KO pFA6 F | TGCAAAAGAAAATAAAAAGAGACAAACAATCATAAACATGCGGATCCCCGGGTTAATTAA | Primer for KO of gene using pFA6 plasmids. |
| MDH3 | MDH3 KO pFA6 R | AGTATAGAGTTAAGAAAAATATAAAAATTGAAGTAGCTCAGAATTCGAGCTCGTTTAAAC | Primer for KO of gene using pFA6 plasmids. |
| MDH3 | MDH3 N' tag CHK R | TTCTTCAAAGTTTCCACAGC | Primer for checking N' tagging of the gene. |
| MDH3 | MDH3 WT CHK F | ATTCAGGGGAAACCATTATC | Primer for testing the presence of the gene. |
| MDH3 | MDH3 WT CHK R | TCGATGGATACTACGCTACC | Primer for testing the presence of the gene. |
| MDH1/2/3 | N 'tag CHK F | CGACAGAGAATTCATCGATG | Primer for checking N' tagging. Using pym plasmid |
| MDH1/2/3 | F2-Rev-Com | TTAATTAACCCGGGGATCCG | Reverse complementary to the F1/F2 primer of the
Longtine's pFA6a plasmids. |