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Validation of FOXO3 as a direct target of miR-155 in HT-29 cells. (A) Results of dual-luciferase assay in HT-29 cells transfected with pmirGLO-FOXO3-3′ UTR-WT or pmirGLO-FOXO3-3′UTR M reporter together with miR-155 mimics or miR control. (B) Predicted miR155-binding sites in the 3′UTR of FOXO3. Data were expressed in mean ± standard devation. miR, microRNA; FOXO3, forkhead box O3; 3′UTR, 3′untranslated region; WT, wild type; M, mutated.
