Figure 4.

Effects of YAP knockdown on MOB2-regulated cell motility in MOB2-knockout SMMC-7721 cells. (A) Reverse transcription-quantitative polymerase chain reaction and (B) western blotting of YAP expression in MOB2 knockout SMMC-7721 cells following transfection with shYAP or shNC. Representative Transwell (C) migration (magnification, ×100) and (D) invasion assays (magnification, ×100). Quantification of results from Transwell (E) migration and (F) invasion assays. Data are presented as the mean ± standard deviation of three independent experiments. *P<0.05 vs. shNC. shRNA, small-hairpin RNA; MOB2, monopolar spindle-one-binder protein 2; shNC, non-targeting shRNA vector; shYAP, YAP-targeting shRNA; YAP, yes-associated protein; shRNA, short hairpin RNA.