Table I.
Antibodies that were used for immunohistochemistry.
| Antibodies | Source r/m | Clone | Company | Dilution | Heat-induced epitope retrieval M/S/E | Vis method |
|---|---|---|---|---|---|---|
| CD3 | m | 2GV6 | Roche | ×1 | S | VENTANA |
| CD5 | m | 4C7 | Leica | ×25 | S | iVIEW |
| DAB Detection kit | ||||||
| VENTANA | ||||||
| Automated immune-histochemistry | ||||||
| CD10 | m | 56C6 | ×40 | S | ||
| CD20cy | m | L26 | DAKO | ×400 | S | |
| MUM-1 | m | MUM1p | ×50 | M | ||
| Bcl-1 (CyclinD1) | m | DSC-6 | ×50 | M | ||
| Bcl-2 | m | 124 | ×100 | S | ||
| Bcl-6 | m | PG-B6 | ×20 | S | ||
| CK7 | m | OV-TL 12/30 | ×100 | E | ||
| CK20 | m | Ks20.8 | ×100 | M | ||
| TTF-1 | m | 8G7G3/1 | ×50 | M | ||
| Pax8 | r | Polyclonal | Proteintech | ×200 | M | |
| ER | ||||||
| Estrogen receptor | m | SP-1 | Roche | ×1 | S | |
| PSA | ||||||
| Prostate-specific | m | ER-PR8 | DAKO | ×100 | M | |
| Antigen | ||||||
| S-100 | r | Polyclonal | ×500 | M | ||
| p40 | r | Polyclonal | Calbiochem | ×500 | S | |
| CDX-2 | m | CDX2-88 | BioGenex | ×50 | S | |
| DOG-1 | m | K9 | Leica | ×100 | S | |
| Chromogranin A | r | Polyclonal | DAKO | ×100 | M | |
| Synaptophysin | m | 27G12 | Nichirei | ×1 | M | |
| CD117, c-kit Oncoprotein | r | Polyclonal | DAKO | ×20 | M | |
| Desmin | m | D33 | ×1 | S |
Heat-induced epitope retrieval M/S/E; CC1/Cell Conditioning Solution (Roche), 95–100°C. M, mild 30 min conditioning; S, standard 60 min Conditioning; E, extended 90 min conditioning. Automated immune-histochemistry; BenchMark XT IHC/ISH Staining Module. Ab, antibody; r/m, rabbit/mouse.