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. 2018 Feb 13;15(4):5977–5982. doi: 10.3892/ol.2018.8038

Figure 2.

Figure 2.

ROCK1 was confirmed as a target of miR-139. (A) Predicted consequential pairing of miR-139 and the target 3′UTR region of ROCK1 (Wt and Mut). (B) BGC-823 and SGC-7901 cells were co-transfected with pGL3-ROCK1-3′UTR-Wt or pGL3-ROCK1-3′UTR-Mut and miR-139 mimic or NC. (C) Reverse transcription-quantitative polymerase chain reaction detection of ROCK1 mRNA expression in BGC-823 and SGC-7901 cells transfected with miR-139 mimic or NC. (D) ROCK1 protein expression was detected by western blot analysis in BGC-823 and SGC-7901 cells transfected with miR-139 mimic or NC. *P<0.05 vs. control. miR-139, microRNA-139; ROCK1, ρ-associated protein kinase 1; hsa, Homo sapiens; Wt, wild-type; Mut, mutant; NC, negative control; UTR, untranslated region.