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. 2018 Mar 6;18:260. doi: 10.1186/s12885-018-4177-0

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Overexpression of TIPE3 with N-terminal flag inhibits the proliferation and migration of lung cancer cells. a Long or short TIPE3 with N-terminal flag was transiently overexpressed in A549 and H1975 cells by plasmid transfection, and detected by western-blot. b, c The growth curves of A549 (b) and H1975 (c) cells overexpressing TIPE3 were determined by CCK8 assay (n = 4 per timepoint). d-f The migration of A549 and H1975 cells overexpressing TIPE3 was determined. Representative (d) and statistic (e, f) data are shown (n = 5 per group). Bars represent mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001