Figure 6.

Mechanism of the apoptosis process in A375 cells. CDK5RAP1 deficiency induces the mistranslation of mitochondrial ms²i⁶A, induced the generation of ROS and the phosphorylation of NF-κB, resulting in apoptosis of A375 cells. PDTC, the inhibitor of NF-κB, prevents the decrease of proliferation and the apoptosis induced by CDK5RAP1 deficiency in A375 cells, but does not affect the generation of ROS in A375 cells, indicating that the ROS pathway is upstream of NF-κB signaling pathway during the apoptosis process. ROS, reactive oxygen species; CDK5RAP1, Cdk5 regulatory subunit-associated protein 1; NF-κB, nuclear factor-κB; PDTC, pyrrolidine dithiocarbamate.