FIGURE 5.

Differential expression of selected genes in response to moderate drought stress in N7001 and TJS2049. Ten μg of total RNA from N7001 or TJS2049 genotypes were analyzed by Northern blot. Controls (Ctrl) correspond to samples from well-irrigated plants, and dehydration (DH) correspond to samples from drought stressed plants, taken when substrate reached a 50% of its water retention capacity (50% FC), usually 3 days after water withdrawal. Clone inserts corresponding to the selected genes were labeled with α ³²P-dCTP and used as hybridization probes. Ethidium bromide staining of rRNA was used to ensure equal loading of RNA samples. The genes and their accession number used as probes are the following: Phototropin 2 (Glyma.08G264900); Histone 2A (Glyma.15G040400); eIFiso4G (Glyma.17G072500); MBF1-like transcription factor (Glyma.06G276200); GT-2 transcription factor (Glyma.10G225200); AN1-like Zinc finger (Glyma.13G341000); NRX (Glyma.06G021200); Dehydrin: DHN- 13 kDa (Glyma.19G114700); Dehydrin: DHN- 27 kDa (Glyma.09G185500); and Glutathione peroxidase: GPX (Glyma.01G219400). The coordinates showing the position of each clone in the dot blot array (Supplementary Figure S1) are marked in parenthesis.