Figure 3.

Sodium Salinomycin, AM251, and aripiprazole reduce human mutant ATXN3 levels in organotypic brain slice cultures from YACMJD84.2 transgenic mice (Q84). (A) Anti-ATXN3 immunoblots show that sodium salinomycin (Na Salinomycin), AM251 and aripiprazole decrease human mutant ATXN3 levels in brainstem and cerebellar fractions of brain slices from hemizygous YACMJD84.2 mice (Q84). Sagittal brain slices (300 μM) from the same mouse were cultured with specific concentrations of the compound or its vehicle at 37°C/5% CO₂. After 48 h of treatment, brainstem and cerebellar regions from each slice were processed separately. Quantification of bands corresponding to human mutant ATXN3 and mouse ATXN3 shown in B and C, respectively. Bars represent the mean percentage of protein relative to levels in vehicle-treated slices and normalized to α-tubulin (±SEM) for three independent experiments using different mice. Comparison between slices treated with a specific compound/concentration and slices treated with vehicle was performed using Student’s t-test with statistical significance, as indicated: *P < 0.05 and **P < 0.01.