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. 2018 Jan 29;15(4):3302–3310. doi: 10.3892/etm.2018.5804

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Figure 6. — (A) Immunohistochemical staining of CD4⁺ in the ovaries (magnification, ×200). (B) CFSE-based proliferative assay for CD4⁺ CD25⁺ Treg lymphocytes. (C) ELISA analysis of IL-10 and IFN-γ expression in the spleen of the three groups. In the control, model and BSHXF-treated groups, the levels of IL-10 were 55.647±9.185, 111.918±11.282 and 85.742±6.166, respectively, and the levels of IFN-γ were 28.831±11.024, 76.844±13.736 and 55.183±11.595, respectively. ***P<0.001 vs. the control group; ^###P<0.001 vs. the model group. BSHXF, Bu Shen Huo Xue formula; IL, interleukin; IFN-γ, interferon-γ.