Figure 5.

Effects of local TGF-β1 levels and dexamethasone treatment on the cytokine concentration in mesenteric lymph nodes of TNBS-treated mice. AdTGF-β1 was delivered to the colons of TNBS mice and dexamethasone (5 mg/kg body weight) was given once a day by orogastric gavage for 4 days. Homogenates of mesenteric lymph nodes were prepared and the levels of cytokines (A) IL-10 and (B) IL-23 were determined using ELISA. Doses: AdTGF-1, 1×10⁷ pfu; AdTGF-2, 1×10⁸ pfu; AdTGF-3, 1×10⁹ pfu. Values are expressed as the mean ± standard deviation (n=4 per group). N.S., no significance; MLN, mesenteric lymph nodes; CTL, control; TNBS, 2,4,6-trinitrobenzenesulfonic acid; AdTGF, adenovirus overexpressing transforming growth factor β1; Dex, dexamethasone; IL, interleukin.