Figure 6.

740Y-P reversed the effects of curcumin on the proliferation and apoptosis of LCSCs. After treatment with curcumin and/or 740Y-P, (A) the proliferation of LCSCs was detected by MTT assay, (B) the apoptosis of LCSCs was detected by flow cytometry, and (C) the percentage of apoptotic cells was calculated. Each experiment was repeated more than three times and the results are demonstrated as the mean ± standard deviation. *P<0.05 and **P<0.01. LCSCs, liver cancer stem cells; ns, non-significant; FITC, fluorescein isothiocyanate; PI, propidium iodide.