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Microscopic images of Alizarin Red staining displaying the formation of calcium nodules in BMSCs-C57 cells following osteogenic culturing for 14 days in (A) CS/DS/rhBMP-2 microsphere group, (B) rhBMP-2 group, (C) CS/DS group and (D) control group. Blue arrows indicate calcium nodules. Scale bars=2 µm. CS, chitosan; DS, dextran sulfate; rhBMP-2, recombinant human bone morphogenetic protein 2.
