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. 2018 Jan;13(1):86–93. doi: 10.4103/1673-5374.224373

Figure 4.

Figure 4

Effects of electroacupuncture (EA) preconditioning on dynamin-related protein 1 (Drp1) expression in focal cerebral ischemia/reperfusion (IR) injury rats.

Total-Drp1 (A) and Mitochondrial Drp1 (Mito-Drp1) (B) at 6, 24 and 48 hours post-reperfusion or sham surgery, as measured by western blot assay and densitometric analysis, and quantified relative to β-actin (Total-Drp1) or cyclooxygenase (Cox)-IV (Mito-Drp1). The lack of expression of the Rho GDP dissociation inhibitor (RhoGDI; a specific cytosolic [but not mitochondrial] biomarker (Francis et al., 2014)) verified the purity of the mitochondrial fraction. Data are presented as the mean ± SD (n = 4 rats per group at each time point), and analyzed by one-way analysis of variance followed by the post hoc Student-Newman-Keuls test. Data are representative of three independent tests. *P < 0.05, **P < 0.01, vs. sham group; #P < 0.05, vs. EA group at the same time point. I: Sham; II: EA 6 hours; III: IR 6 hours; IV: EA 24 hours; V: IR 24 hours; VI: EA 48 hours; VII: IR 48 hours.